Direct spectrophotometric method for measurement of bilirubin in newborns: comparison with HPLC and an automated diazo method.

نویسندگان

  • Steven C Kazmierczak
  • Alex F Robertson
  • Paul G Catrou
  • Kimberly P Briley
  • Bill L Kreamer
  • Glenn R Gourley
چکیده

Bilirubin in Newborns: Comparison with HPLC and an Automated Diazo Method, Steven C. Kazmierczak, Alex F. Robertson, Paul G. Catrou, Kimberly P. Briley, Bill L. Kreamer, and Glenn R. Gourley ( Department of Pathology, Oregon Health and Sciences University, 3181 SW Sam Jackson Park Rd., Portland, OR 97201; Departments of 2 Pediatrics and 3 Pathology and Laboratory Medicine, Brody School of Medicine of East Carolina University, Greenville, NC 27858; 4 Department of Pediatrics and Waisman Center, University of Wisconsin, 1500 Highland Ave., Madison, WI 53705; * address correspondence to this author at: Department of Pathology, Oregon Health and Sciences University, Mailcode L471, 3181 SW Sam Jackson Park Rd., Portland, OR 97201; fax 503-494-8148, e-mail [email protected])

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Automated total and neonatal bilirubin values in newborns: is a distinction clinically relevant?

Guidelines for managing hyperbilirubinemia in newborns were developed by using diazo methods that measure total and direct-reacting bilirubins and calculate an indirect fraction. The automated Kodak Ektachem system allows for measuring serum bilirubin by either of two dry-slide methods: TBIL, involving a modified diazo method, and NBIL, involving a dual-wavelength colorimetric method that fract...

متن کامل

Light has a greater effect on direct bilirubin measured by the bilirubin oxidase method than by the diazo method.

We compared the effect of light on direct-reacting bilirubin (DBIL) measurement by the bilirubin oxidase (EC 1.3.3.5; BOX) method and by the Jendrassik-Gróf diazo method. DBIL concentrations determined by the BOX method in the sera of hyperbilirubinemic infants treated with phototherapy yielded falsely higher values than those by the direct diazo method. A similar tendency was noted when DBIL c...

متن کامل

Comparison of direct spectrophotometric determinations of bilirubin with candidate reference method in sera of newborns.

Bilirubin was determined by direct spectrophotometry and by the candidate reference method of Doumas et al. (Clin. Chem. (1985) 31; 1789-1798) in a group of more than 230 newborns, and the results were compared. Direct spectrophotometry by Vink et al. (Clin. Chem. (1988) 34; 67-70) gives the following regression equation in comparison with the reference method: y = 0.967x + 6.57 with r = 0.9874...

متن کامل

Spectrophotometric Method for Hydrogen Peroxide Determination through Oxidation of Organic Dyes

Hydrogen peroxide is an important mild oxidant which is widely used in various applications including remediation of waste waters and drinking water resources. The safe level of H2O2 residue has to be controlled through accurate and precise measurement methods. A facile spectrophotometric method for the determination of hydrogen peroxide is based on the reaction of iodine ...

متن کامل

Modification of the DuPont aca as exemplified by a new procedure for neonatal total bilirubin.

Hemoglobin interferes with determination of total bilirubin by the diazo procedure with the DuPont aca. We compared results by that procedure with those by a manual direct spectrophotometric two-wavelength method. Because the manual method was not inhibited by hemolysis as was the DuPont aca diazo procedure, we reprogrammed our DuPont aca to measure the absorbance of diluted serum samples at 45...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Clinical chemistry

دوره 48 7  شماره 

صفحات  -

تاریخ انتشار 2002